Rat Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Name: Rat Apolipoprotein E/ApoE ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-82119

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82119

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Apolipoprotein E/ApoE in samples. No significant cross-reactivity or interference between Rat Apolipoprotein E/ApoE and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6%

Inter-Assay Precision (Precision between assays) CV% < 6%

Recovery for Rat Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Recovery

average = 97% for serum; average = 98% for EDTA plasma; average = 90% for cell culture media

Linearity

Scientific Data Images for Rat Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

ELISA: Rat Apolipoprotein E/ApoE ELISA Kit (Colorimetric) [NBP2-82119] -

ELISA: Rat Apolipoprotein E/ApoE ELISA Kit (Colorimetric) [NBP2-82119] - Standard Curve Reference

Kit Contents for Rat Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein E/ApoE

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Alternate Names

APOE

Gene Symbol