Human APRIL/TNFSF13 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82419

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL )

Sensitivity

0.4 ng/mL

Assay Range

0.78 - 50.0 ng/mL

View all APRIL/TNFSF13 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human APRIL positive serum. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.1%

Inter-Assay Precision (Precision between assays) CV% < 7.1%

Recovery for Human APRIL/TNFSF13 ELISA Kit (Colorimetric)

Recovery

The amount of endogenous human APRIL in unspiked serum was subtracted from the spike values. The overall mean recovery was 76%.

Scientific Data Images for Human APRIL/TNFSF13 ELISA Kit (Colorimetric)

ELISA: Human APRIL/TNFSF13 ELISA Kit (Colorimetric) [NBP1-82419]

ELISA: Human APRIL/TNFSF13 ELISA Kit (Colorimetric) [NBP1-82419]

ELISA: Human APRIL/TNFSF13 ELISA Kit (Colorimetric) [NBP1-82419] - Representative standard curve for human APRIL ELISA. Human APRIL was diluted in serial 2-fold steps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed.

Kit Contents for Human APRIL/TNFSF13 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human APRIL
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human APRIL polyclonal antibody
  • Human APRIL Standard lyophilized, 100 ng/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: APRIL/TNFSF13

APRIL (A Proliferation-Inducing Ligand) is a member of the tumor necrosis factor family. APRIL shows high levels of expression in tumors of different origin and low level of expression in normal cells. APRIL shares two TNF receptor family members, TACI and BCMA with another TNF homolog, BlyS/BAFF both of which have been reported to play a role in autoimmune disease and cancer. APRIL appears to play a role in T-independent type II antigen responses and T cell survival, but can also induce proliferation/survival of non lymphoid cells. Local production of APRIL was found in arthritic joints of patients with inflammatory arthritis. Biologically active BlyS/BAFF and APRIL heterotrimers are expressed in patients with systemic immune based rheumatic diseases. A soluble form of the high affinity BCMA receptor has been shown to inhibit the proliferative activity of APRIL in vitro, thus decreasing tumor cell proliferation, while APRIL-transfected cells show an increased rate of tumor growth very directly, suggesting that APRIL is implicated in the regulation of tumor cell growth.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2

Gene Symbol

TNFSF13

Additional APRIL/TNFSF13 Products

Product Documents for Human APRIL/TNFSF13 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human APRIL/TNFSF13 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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