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Human ATF2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69881

Novus Biologicals, part of Bio-Techne
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NBP2-69881

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human ATF2 in samples. No significant cross-reactivity or interference between Human ATF2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.69%

Inter-Assay Precision (Precision between assays) CV% < 5.28%

Recovery for Human ATF2 ELISA Kit (Colorimetric)

Recovery

89-109%

Linearity

Human ATF2 ELISA Kit (Colorimetric)

Scientific Data Images for Human ATF2 ELISA Kit (Colorimetric)

Human ATF2 ELISA Kit (Colorimetric)

ELISA: Human ATF2 ELISA Kit (Colorimetric) [NBP2-69881] -

ELISA: Human ATF2 ELISA Kit (Colorimetric) [NBP2-69881] - Standard Curve Reference

Kit Contents for Human ATF2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for Human ATF2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human ATF2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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