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Mouse Axin-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69904

Novus Biologicals, part of Bio-Techne
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NBP2-69904

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse AXIN2 in samples. No significant cross-reactivity or interference between Mouse AXIN2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.4%

Inter-Assay Precision (Precision between assays) CV% < 4.59%

Recovery for Mouse Axin-2 ELISA Kit (Colorimetric)

Recovery

84-103%

Linearity

Mouse Axin-2 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Axin-2 ELISA Kit (Colorimetric)

Mouse Axin-2 ELISA Kit (Colorimetric)

ELISA: Mouse Axin-2 ELISA Kit (Colorimetric) [NBP2-69904] -

ELISA: Mouse Axin-2 ELISA Kit (Colorimetric) [NBP2-69904] - Standard Curve Reference

Kit Contents for Mouse Axin-2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Axin-2

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. (provided by RefSeq)

Long Name

Axis Inhibitor 2

Alternate Names

AXIL, Axin2, Conductin, MGC10366, MGC126582

Gene Symbol

AXIN2

Additional Axin-2 Products

Product Documents for Mouse Axin-2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Axin-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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