Mouse B7-1/CD80 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0991

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Tissue Lysate (100 uL)

Sensitivity

<10 pg/mL

Assay Range

62.5 to 4000 pg/mL

View all B7-1/CD80 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Description

Quality control test: Standard curve. Cd80 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Cd80.

Kit Contents for Mouse B7-1/CD80 ELISA Kit (Colorimetric)

  • ABC diluent buffer: 12ml.
  • Antibody diluent buffer: 12ml.
  • Avidin-Biotin-Peroxidase Complex (ABC): 130µl, dilution 1:100.
  • Biotinylated anti- mouse CD80 antibody: 130µl, dilution 1:100.
  • Lyophilized recombinant mouse CD80 standard: 10ng/tube×2.
  • One 96-well plate precoated with anti- mouse CD80 antibody.
  • Sample diluent buffer: 30 ml
  • TMB color developing agent: 10ml.
  • TMB stop solution: 10ml.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B7-1/CD80

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for Mouse B7-1/CD80 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse B7-1/CD80 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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