Human BACE-1 ELISA Kit (Chemiluminescence)

Name: Human BACE-1 ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP2-66742

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66742

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.09 ng/mL

Assay Range

0.16-10 ng/mL

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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human BACE1 in samples. No significant cross-reactivity or interference between Human BACE1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.52%

Inter-Assay Precision (Precision between assays) CV% < 10.2%

Recovery for Human BACE-1 ELISA Kit (Chemiluminescence)

Recovery

84-110%

Linearity

Scientific Data Images for Human BACE-1 ELISA Kit (Chemiluminescence)

ELISA: Human BACE-1 ELISA Kit (Chemiluminescence) [NBP2-66742] -

ELISA: Human BACE-1 ELISA Kit (Chemiluminescence) [NBP2-66742] - Standard Curve Reference

Kit Contents for Human BACE-1 ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BACE-1

BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 1

Alternate Names

BACE1

Gene Symbol