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Human BACH1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18220

Novus Biologicals, part of Bio-Techne
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NBP3-18220

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and cell culture supernates (100 ul)

Sensitivity

22.15 pg/ml

Assay Range

46.87-3000 pg/ml

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human BACH1 in the sample, this Human BACH1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human BACH1 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 15

Recovery for Human BACH1 ELISA Kit (Colorimetric)

Recovery

81-113%

Scientific Data Images for Human BACH1 ELISA Kit (Colorimetric)

ELISA: Human BACH1 ELISA Kit (Colorimetric) [NBP3-18220]

ELISA: Human BACH1 ELISA Kit (Colorimetric) [NBP3-18220]

ELISA: Human BACH1 ELISA Kit (Colorimetric) [NBP3-18220] - Standard Curve Reference

Kit Contents for Human BACH1 ELISA Kit (Colorimetric)

  • Balance Solution
  • Enzyme Conjugate
  • Microtiter Plate
  • Standards
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BACH1

BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.

Long Name

BTB and CNC homolog 1

Alternate Names

BTB, HA2303

Gene Symbol

BACH1

Additional BACH1 Products

Product Documents for Human BACH1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human BACH1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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