Human BAK ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69930

Novus Biologicals, part of Bio-Techne
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NBP2-69930
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63 - 40 ng/mL

View all BAK ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human BAK1 in samples. No significant cross-reactivity or interference between Human BAK1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.74%

Inter-Assay Precision (Precision between assays) CV% < 4.72%

Recovery for Human BAK ELISA Kit (Colorimetric)

Recovery

88-104%

Linearity

Human BAK ELISA Kit (Colorimetric)

Scientific Data Images for Human BAK ELISA Kit (Colorimetric)

Human BAK ELISA Kit (Colorimetric)

ELISA: Human BAK ELISA Kit (Colorimetric) [NBP2-69930] -

ELISA: Human BAK ELISA Kit (Colorimetric) [NBP2-69930] - Standard Curve Reference

Kit Contents for Human BAK ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BAK

Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis.

Long Name

Bcl-2 Antagonist/Killer

Alternate Names

BAK1, BCL2L7

Gene Symbol

BAK1

Additional BAK Products

Product Documents for Human BAK ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human BAK ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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