Rat Bcl-xL ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77043

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and cell lysates (50 - 100 uL)

Sensitivity

11.72 pg/mL

Assay Range

46.88 pg/mL - 3000 pg/mL

View all Bcl-xL ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat Bcl-2. No significant cross-reactivity or interference between Rat Bcl-2 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Recovery for Rat Bcl-xL ELISA Kit (Colorimetric)

Recovery

88 - 102%

Scientific Data Images for Rat Bcl-xL ELISA Kit (Colorimetric)

Rat Bcl-xL ELISA Kit (Colorimetric)

Rat Bcl-xL ELISA Kit (Colorimetric) [NBP2-77043] -

Rat Bcl-xL ELISA Kit (Colorimetric) [NBP2-77043] - Standard Curve Reference

Kit Contents for Rat Bcl-xL ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay Plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction Manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Bcl-xL

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Long Name

B Cell Lymphoma/Leukemia x Long Form

Alternate Names

BclxL

Gene Symbol

BCL2L1

Additional Bcl-xL Products

Product Documents for Rat Bcl-xL ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Bcl-xL ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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