Mouse BDNF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0331

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Cell Lysates, Plasma (Citrate, EDTA, Heparin), Serum (100 uL)

Sensitivity

2 pg/mL

Assay Range

31.2 to 2000 pg/mL

View all BDNF ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Description

Bdnf (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Bdnf.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.7%

Inter-Assay Precision (Precision between assays) CV% < 7.2%

Kit Contents for Mouse BDNF ELISA Kit (Colorimetric)

  • 96-well Plate precoated with anti-mouse BDNF antibody
  • ABC diluent buffer
  • Adhesive cover
  • Antibody diluent buffer
  • Avidin-Biotin-Peroxidase Complex (ABC) (dilution 1:100)
  • Biotinylated anti- mouse BDNF antibody (dilution 1:100)
  • Lyophilized recombinant mouse BDNF standard
  • Sample diluent buffer
  • TMB color developing agent
  • TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BDNF

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family. BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Gene Symbol

BDNF

Additional BDNF Products

Product Documents for Mouse BDNF ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse BDNF ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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