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Human beta-NGF ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62775

Novus Biologicals, part of Bio-Techne
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NBP2-62775

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

4.69 pg/mL

Assay Range

7.81-500 pg/mL

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.85%

Inter-Assay Precision (Precision between assays) CV% < 8.85%

Recovery for Human beta-NGF ELISA Kit (Chemiluminescence)

Recovery

97-113%

Linearity

Human beta-NGF ELISA Kit (Chemiluminescence)

Scientific Data Images for Human beta-NGF ELISA Kit (Chemiluminescence)

Human beta-NGF ELISA Kit (Chemiluminescence)

ELISA: Human beta-NGF ELISA Kit (Chemiluminescence) [NBP2-62775] -

ELISA: Human beta-NGF ELISA Kit (Chemiluminescence) [NBP2-62775] - Standard Curve Reference

Kit Contents for Human beta-NGF ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: beta-NGF

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Long Name

beta Nerve Growth Factor

Alternate Names

betaNGF, NGFB

Gene Symbol

NGF

Additional beta-NGF Products

Product Documents for Human beta-NGF ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human beta-NGF ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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