Mouse beta-NGF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81189

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

View all beta-NGF ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse beta-NGF in samples. No significant cross-reactivity or interference between Mouse beta-NGF and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.08%

Inter-Assay Precision (Precision between assays) CV% < 5.41%

Recovery for Mouse beta-NGF ELISA Kit (Colorimetric)

Recovery

86-106%

Linearity

Mouse beta-NGF ELISA Kit (Colorimetric)

Scientific Data Images for Mouse beta-NGF ELISA Kit (Colorimetric)

Mouse beta-NGF ELISA Kit (Colorimetric)

ELISA: Mouse beta-NGF ELISA Kit (Colorimetric) [NBP2-81189] -

ELISA: Mouse beta-NGF ELISA Kit (Colorimetric) [NBP2-81189] - Standard Curve Reference

Kit Contents for Mouse beta-NGF ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: beta-NGF

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Long Name

beta Nerve Growth Factor

Alternate Names

betaNGF, NGFB

Gene Symbol

NGF

Additional beta-NGF Products

Product Documents for Mouse beta-NGF ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse beta-NGF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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