BMP-2 ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DBP200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Bone Extracts (50 µL)
Sensitivity
29 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Bone Extracts)
Product Summary for BMP-2 Quantikine ELISA Kit
The Quantikine Human BMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-2 in bone tissue extracts and cell culture supernates. It contains CHO cell-expressed recombinant human BMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human, Mouse, Rat
Specificity
Natural and mammalian-expressed recombinant human BMP-2. This assay does not recognize E. coli-expressed BMP-2.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Sample Values
Cell Culture Supernates - Cell culture supernates from the following unstimulated cell lines
were tested for natural BMP-2. No BMP-2 was detected.
| Cell Lines | Type | Growth Conditions |
| ATDC5 | Mouse chondrogenic | :1 mixture of F-12 and DMEM + 5% FBS, L-glutamine, penicillin, and streptomycin |
| MCF-7 | Human breast cancer | 1:1 mixture of high glucose DMEM and F-12 + 10% FBS, L-glutamine, penicillin, and streptomycin |
| MDA-MB-453 | Human breast cancer | RPMI + 10% FBS, L-glutamine, penicillin, and streptomycin |
| ST-2 | Mouse-bone marrow stroma-cell derived | RPMI + 10% FBS, L-glutamine, penicillin, and streptomycin |
| U2OS | Human osteosarcoma | Grown to confluency in McCoy's 5a media + 15% FBS, L-glutamine, penicillin, and streptomycin |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Bone Extracts, Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 323 | 1010 | 2313 | 344 | 1043 | 2213 |
| Standard Deviation | 8.3 | 28.3 | 56.0 | 25.2 | 55.4 | 140.2 |
| CV% | 2.6 | 2.8 | 2.4 | 7.3 | 5.3 | 6.3 |
Recovery for BMP-2 Quantikine ELISA Kit
The recovery of recombinant human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| 2 M Guanidine-HCI (n=1) | 98 | 94-101 |
| 2 M Urea (n=1) | 101 | 92-106 |
| Cell Culture Media (n=4) | 102 | 94-110 |
Linearity
The following samples were spiked with rhBMP-2 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess the linearity of the assay.
Scientific Data Images for BMP-2 Quantikine ELISA Kit
Human/Mouse/Rat BMP-2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BMP-2
BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.
Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.
Long Name
Bone Morphogenetic Protein 2
Alternate Names
BDA2, BMP2, SSFSC
Gene Symbol
BMP2
Additional BMP-2 Products
Product Documents for BMP-2 Quantikine ELISA Kit
Product Specific Notices for BMP-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.