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Human C-Reactive Protein/CRP ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0238

Novus Biologicals, part of Bio-Techne
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KA0238

Key Product Details

Sample Type & Volume Required Per Well

Serum (10 uL)

Sensitivity

0.1 mg/L

Assay Range

0 to 0.10 mg/L

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Quality control test: Standard curve. CRP (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of C- reactive protein.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.44%

Inter-Assay Precision (Precision between assays) CV% < 3.28%

Recovery for Human C-Reactive Protein/CRP ELISA Kit (Colorimetric)

Recovery

100.40%

Scientific Data Images for Human C-Reactive Protein/CRP ELISA Kit (Colorimetric)

ELISA: Human C-Reactive Protein/CRP ELISA Kit (Colorimetric) [KA0238]

ELISA: Human C-Reactive Protein/CRP ELISA Kit (Colorimetric) [KA0238]

ELISA: C-Reactive Protein/CRP ELISA Kit [HRP] [KA0238] - Standard curve

Kit Contents for Human C-Reactive Protein/CRP ELISA Kit (Colorimetric)

  • Antibody-Coated Wells: Microtiter wells coated with mouse monoclonal anti-CRP.
  • CRP Enzyme Conjugate Reagent: Contains goat anti-CRP conjugated to horseradish peroxidase with preservatives.
  • hsCRP Sample Diluent: Contains phosphate buffer-BSA solution with preservatives.
  • Reference Standard Set: Contains 0, 0.005, 0.010, 0.025, 0.050 and 0.100 mg/L CRP in serum based buffer with preservatives.
  • Stop Solution (1N HCl)
  • TMB Reagent (One-Step)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: C-Reactive Protein/CRP

C Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture.

Alternate Names

CRP

Gene Symbol

CRP

Additional C-Reactive Protein/CRP Products

Product Documents for Human C-Reactive Protein/CRP ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human C-Reactive Protein/CRP ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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