Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59963

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

3.8 U/mL

Assay Range

10 U/mL - 150 U/mL

View all CA19-9 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human CA19-9. No significant cross-reactivity or interference between human CA19-9 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 20

Scientific Data Images for Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric)

Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric) [NBP2-59963] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed
Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric)

Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric) [NBP2-59963] -

Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric) [NBP2-59963] - Standard Curve Reference

Kit Contents for Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CA19-9

This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antibody specific for CA19-9. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CA19-9 and incubated. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA19-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Carbohydrate Antigen 19-9

Alternate Names

CA 19-9

Additional CA19-9 Products

Product Documents for Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CA19-9/Sialyl Lewis A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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