Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75045

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all Calcium-sensing R/CaSR ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human CaSR in samples. No significant cross-reactivity or interference between Human CaSR and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.49%

Inter-Assay Precision (Precision between assays) CV% < 4.81%

Recovery for Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

Recovery

89-104%

Linearity

Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

Scientific Data Images for Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

ELISA: Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric) [NBP2-75045] -

ELISA: Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric) [NBP2-75045] - Standard Curve Reference

Kit Contents for Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Calcium-sensing R/CaSR

Calcium-Sensing Receptor (CaSR) is a member of the G protein-coupled receptor family. CaSR is an integral membrane protein that senses changes in the extracellular concentration of calcium ions and controls the release of parathyroid hormone. The activity of the calcium-sensing receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.

Mutations that inactivate CaSR lead to familial hypocalciuric hypercalcemia, whereas mutations that activate CaSR cause autosomal dominant hypocalcemia. Calcium-Sensing Receptor antibodies are useful tools for parathyroid studies and research on cellular calcium homeostasis regulation.

Long Name

Calcium-sensing Receptor

Alternate Names

CAR, CaSR, EIG8, FHH, FIH, GPRC2A, HHC1, NSHPT, PCaR1

Gene Symbol

CASR

Additional Calcium-sensing R/CaSR Products

Product Documents for Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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