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Human Calretinin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06812

Novus Biologicals, part of Bio-Techne
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NBP3-06812

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Calretinin in samples. No significant Calretininoss-reactivity or interference between Human Calretinin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.42

Inter-Assay Precision (Precision between assays) 5.36

Recovery for Human Calretinin ELISA Kit (Colorimetric)

Recovery

87 - 103 %

Linearity

Human Calretinin ELISA Kit (Colorimetric)

Scientific Data Images for Human Calretinin ELISA Kit (Colorimetric)

Human Calretinin ELISA Kit (Colorimetric)

ELISA: Human Calretinin ELISA Kit (Colorimetric) [NBP3-06812] -

ELISA: Human Calretinin ELISA Kit (Colorimetric) [NBP3-06812] - Standard Curve Reference

Kit Contents for Human Calretinin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

Additional Calretinin Products

Product Documents for Human Calretinin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Calretinin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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