Canine CCL2/MCP-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # CACP00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
10.8 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Canine CCL2/MCP-1 Quantikine ELISA Kit
The Quantikine Canine MCP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine MCP-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine MCP-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine MCP-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Canine
Specificity
Natural and recombinant canine MCP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 43 | 43 | 43 |
| Mean (pg/mL) | 37.1 | 144 | 632 | 42.7 | 133 | 655 |
| Standard Deviation | 2.2 | 5.8 | 22 | 5.4 | 7.5 | 30 |
| CV% | 5.9 | 4.0 | 3.5 | 12.6 | 5.6 | 4.6 |
Recovery for Canine CCL2/MCP-1 Quantikine ELISA Kit
The recovery of canine MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 112 | 106-121 |
| EDTA Plasma (n=4) | 110 | 102-116 |
| Heparin Plasma (n=4) | 103 | 96-112 |
| Serum (n=4) | 93 | 84-102 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of canine MCP-1 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Canine CCL2/MCP-1 Quantikine ELISA Kit
Canine CCL2/JE/MCP-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL2/JE/MCP-1
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
Additional CCL2/JE/MCP-1 Products
Product Documents for Canine CCL2/MCP-1 Quantikine ELISA Kit
Product Specific Notices for Canine CCL2/MCP-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.