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Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59964

Novus Biologicals, part of Bio-Techne
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NBP2-59964-1Kit

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernates, ascitic fluid, tissue homogenates and cell lysates (50 - 100 uL)

Sensitivity

2.5 U/mL

Assay Range

10 U/mL - 120 U/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human CA50. No significant cross-reactivity or interference between human CA50 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 20

Scientific Data Images for Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric)

Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric)

Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric) [NBP2-59964] -

Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric) [NBP2-59964] - Standard Curve Reference

Kit Contents for Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Carbohydrate Antigen 50

This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antibody specific for CA50. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CA50 and incubated. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA50 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

CA50

Additional Carbohydrate Antigen 50 Products

Product Documents for Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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