Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70052

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all Carbonic Anhydrase I/CA1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CA1 in samples. No significant cross-reactivity or interference between Rat CA1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.15%

Inter-Assay Precision (Precision between assays) CV% < 5.23%

Recovery for Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric)

Recovery

84-105%

Linearity

Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric)

Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric)

ELISA: Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70052] -

ELISA: Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric) [NBP2-70052] - Standard Curve Reference

Kit Contents for Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Carbonic Anhydrase I/CA1

Carbonic anhydrase performs an important role in respiration by facilitating transfer of CO2. Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.

Alternate Names

CA1

Gene Symbol

CA1

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Carbonic Anhydrase I/CA1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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