Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric)

Name: Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-70053

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70053

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human CA12 in samples. No significant cross-reactivity or interference between Human CA12 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.68%

Inter-Assay Precision (Precision between assays) CV% < 5.9%

Recovery for Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric)

Recovery

86-104%

Linearity

Scientific Data Images for Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric)

ELISA: Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric) [NBP2-70053] -

ELISA: Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric) [NBP2-70053] - Standard Curve Reference

Kit Contents for Human Carbonic Anhydrase XII/CA12 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Carbonic Anhydrase XII/CA12

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Alternate Names

CA12

Gene Symbol