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Human Caspase-6 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75028

Novus Biologicals, part of Bio-Techne
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NBP2-75028

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human CASP6 in samples. No significant cross-reactivity or interference between Human CASP6 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.43%

Inter-Assay Precision (Precision between assays) CV% < 5.61%

Recovery for Human Caspase-6 ELISA Kit (Colorimetric)

Recovery

92-110%

Linearity

Human Caspase-6 ELISA Kit (Colorimetric)

Scientific Data Images for Human Caspase-6 ELISA Kit (Colorimetric)

Human Caspase-6 ELISA Kit (Colorimetric)

ELISA: Human Caspase-6 ELISA Kit (Colorimetric) [NBP2-75028] -

ELISA: Human Caspase-6 ELISA Kit (Colorimetric) [NBP2-75028] - Standard Curve Reference

Kit Contents for Human Caspase-6 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Caspase-6

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Alternate Names

CASP6, Caspase6, Mch2

Gene Symbol

CASP6

Additional Caspase-6 Products

Product Documents for Human Caspase-6 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Caspase-6 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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