Mouse CCL11/Eotaxin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92660

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

3.6 pg/mL

Assay Range

31.3 - 2,000 pg/mL

View all CCL11/Eotaxin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.60%

Inter-Assay Precision (Precision between assays) CV% < 9.40%

Recovery for Mouse CCL11/Eotaxin ELISA Kit (Colorimetric)

Recovery

The unspiked serum was used as blank in these experiments. The overall mean recovery was 66%.

Scientific Data Images for Mouse CCL11/Eotaxin ELISA Kit (Colorimetric)

Mouse CCL11/Eotaxin ELISA Kit [NBP1-92660] - Data for Mouse Eotaxin Platinum ELISA

Kit Contents for Mouse CCL11/Eotaxin ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse Eotaxin
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse Eotaxin polyclonal antibody
  • Mouse Eotaxin Standard lyophilized, 4 ng/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CCL11/Eotaxin

Eotaxin is a member of the CC chemokine family of inflammatory and immunoregulatory cytokines. Constitutive Eotaxin mRNA expression has been detected in multiple tissues, most often it appears to be induced by inflammatory cytokines such as IL-1, TNF-alpha and IFN-gamma in fibroblasts, endothelial and epithelial cells. Following allergen challenge Eotaxin mRNA has been shown to be rapidly up-regulated in airway epithelium an alveolar macrophages. Eotaxin expression has also been detected in smooth muscle cells, chondrocytes and eosinophils. Mouse Eotaxin activity is mediated by the mouse CC chemokine receptor CCR3, which, unlike human CCR3, can also be activated by mouse MIP-1alpha. Among CC chemokine familiy members, Eotaxin is functionally and structurally mostly related to the MCP/Eotaxin proteins. Mouse Eotaxin cDNA encodes a 97 amino acid residue precursor protein that is cleaved to generate the 74 aa residue mature protein. Mouse Eotaxin has been shown to be a potent chemoattractant for eosinophils during inflammation and allergic reactions and to be involved in the growth of myeloid cell progenitors and the differentiation of mast cells during embryonic development. A role in numerous eosinophil-associated gastrointestinals disorders as food allergy, parasitic infections, allergic colitis and inflammatory Bowel disease has been described.

Alternate Names

Eotaxin

Gene Symbol

CCL11

Additional CCL11/Eotaxin Products

Product Documents for Mouse CCL11/Eotaxin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse CCL11/Eotaxin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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