Rabbit CCL2/JE/MCP-1 ELISA Kit (Chemiluminescence)

Name: Rabbit CCL2/JE/MCP-1 ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP3-42405

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42405

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Product Datasheet / COA

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Product Specifications
Precision
Recovery
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids (100 uL)

Sensitivity

19.1 pg/mL

Assay Range

54.9 - 40000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Rabbit

Description

Assay time: 2 hours, 40 minutes

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery for Rabbit CCL2/JE/MCP-1 ELISA Kit (Chemiluminescence)

Recovery

87 - 100%

Kit Contents for Rabbit CCL2/JE/MCP-1 ELISA Kit (Chemiluminescence)

Assay Diluent A
Assay Diluent B
Detection Reagent A
Detection Reagent B
Instruction manual
Plate sealer for 96 wells
Pre-coated ready to use 96-well strip plate
Standard
Standard Diluent
Substrate A
Substrate B
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CCL2/JE/MCP-1

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Gene Symbol

CCL2

Additional CCL2/JE/MCP-1 Products

Product Documents for Rabbit CCL2/JE/MCP-1 ELISA Kit (Chemiluminescence)

Product Datasheets

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COA

Product Specific Notices for Rabbit CCL2/JE/MCP-1 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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