CCL20/MIP-3 alpha ELISA Development Kit

Name: CCL20/MIP-3 alpha ELISA Development Kit
Brand: Novus Biologicals
SKU: DDXK-E-MIP-3-alpha

Novus Biologicals, part of Bio-Techne | Catalog # DDXK-E-MIP-3-alpha

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

0.01 - 20ng/ml

Sample Type

Human cell culture supernatants, plasma, serum, and various biological fluids (100 uL)

Sensitivity

0.2ng/ml

Reactivity

Human

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Product Specifications

Sample Volume Required

100 uL

Conjugate

Unconjugated

Application Notes

The human MIP-3 alpha ELISA kit is for quantitative determination of MIP3 alpha concentrations in human cell culture supernatants and biological fluids (plasma, serum). This kit is for research purpose only.

Scientific Data Images for CCL20/MIP-3 alpha ELISA Development Kit

ELISA: CCL20/MIP-3 alpha ELISA Development Kit [DDXK-E-MIP-3-alpha]

ELISA: CCL20/MIP-3 alpha ELISA Kit [DDXK-E-MIP-3-alpha] - Analysis of MIP-3alpha secretion by CHA cell line cultured in the presence of increasing doses of IL1-beta

Kit Contents for CCL20/MIP-3 alpha ELISA Development Kit

Capture Antibody: 0.5 mg/mL of mouse anti-MIP3 alpha monoclonal antibody (clone 319F6).
Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-MIP3 alpha mouse monoclonal antibody (clone 206D9).
Standard: Each vial contains 1 g/mL of recombinant human MIP3 alpha produced, purified and concentrated from eukaryotic cells. A 7-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20 ng /mL is recommende

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CCL20/MIP-3 alpha

Macrophage inflammatory protein (MIP-3a)/ CCL20 is a CC-type chemokine mapped to chromosome 2 in humans. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium. MIP-3a plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3a is the most potent chemokine inducing the selective migration of in vitro-generated CD34+ hematopoietic progenitor cell-derived LC precursors and skin LCs, in accordance with the restricted MIP-3a receptor (CC chemokine receptor 6) expression by these cells. (b) MIP-3a is mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes is blocked by an antibody against MIP-3a. (c) In vivo, MIP-3a is selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP-3a up regulation is associated with an increase in LC turnover. (d) The secretion of MIP-3a is strongly up regulated by cells of epithelial origin after inflammatory stimuli (interleukin 1b plus TNFa) or T cell signals (Homey B. et al, 2000, J.Immunol. 164:6621-32). Monoclonal antibodies anti-MIP3a were obtained after mice immunization with MIP-3a-transfected eukaryotic cells. These clones were used to set up a human MIP-3a specific Elisa, which also detect macaque MIP3a. No cross-reactivity with MIP-1a, MIP-1b, MIP-1d, MIP-3b, eotaxin, 6Ckine, Rantes, MCP-1, TARC, MDC, TECK, SDF-1 and IP-10 was detected (Dieu-Nosjean et al, J;Exp.med, 2000 192: 705-18).

Alternate Names

exodus-1, LARC, MIP-3 alpha, MIP3 alpha

Gene Symbol

CCL20

Additional CCL20/MIP-3 alpha Products

Product Documents for CCL20/MIP-3 alpha ELISA Development Kit

Product Datasheets

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Protocols & Manuals

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COA

Product Specific Notices for CCL20/MIP-3 alpha ELISA Development Kit

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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