Human CD117/c-kit ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14627

Novus Biologicals, part of Bio-Techne
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NBP3-14627
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell culture supernatant (100 ul)

Sensitivity

0.095 ng/ml

Assay Range

0.312-10 ng/ml

View all CD117/c-kit ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

Recognizes both natural and recombinant human soluble CD117/c-kit. No cross reactivity with other human soluble receptors.

Description

Incubation: 1 hours 45 minutes

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6

Inter-Assay Precision (Precision between assays) CV% < 7.9

Scientific Data Images for Human CD117/c-kit ELISA Kit (Colorimetric)

ELISA: Human CD117/c-kit ELISA Kit (Colorimetric) [NBP3-14627]

ELISA: Human CD117/c-kit ELISA Kit (Colorimetric) [NBP3-14627]

ELISA: Human CD117/c-kit ELISA Kit (Colorimetric) [NBP3-14627] - Example of Human CD117/c-kit Standard Curve.

Kit Contents for Human CD117/c-kit ELISA Kit (Colorimetric)

  • Biotinylated secondary antibody
  • Buffers
  • Controls (where applicable)
  • Detailed procedure.
  • Pre-coated strip plates
  • Standards
  • Stop reagents
  • Streptavidin-HRP
  • TMB

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

Additional CD117/c-kit Products

Product Documents for Human CD117/c-kit ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CD117/c-kit ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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