Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88106

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-88106
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL)

Sensitivity

0.27 ng/mL

Assay Range

0.31 - 20.0 ng/mL

View all CD25/IL-2R alpha ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay detects soluble IL-2R in human serum, plasma, buffered solution, or cell culture medium. It will exclusively recognize both natural and recombinant human IL-2R.

Description

The human sIL-2R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sIL-2R

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.2%

Inter-Assay Precision (Precision between assays) CV% < 9.8%

Recovery for Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

Recovery

75%

Scientific Data Images for Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

ELISA: Human CD25/IL-2R alpha ELISA Kit (Colorimetric) [NBP1-88106]

ELISA: Human CD25/IL-2R alpha ELISA Kit (Colorimetric) [NBP1-88106]

ELISA: CD25/IL-2 R alpha ELISA Kit [NBP1-88106] - Standard curve

Kit Contents for Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human GRO alpha (CXCL1)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD25/IL-2R alpha

IL-2 has been described as a factor that promotes the growth and proliferation of human T-cells thus occupying a pivotal role in the generation of the immune response. This proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific cell surface receptor following T lymphocyte activation. The receptor for IL-2 is composed of at least three distinct polypeptide subunits , the IL-2R-alpha, IL-2R-beta, and the IL-2R-gamma chains giving rise to a 55-65 kD membrane bound protein. In addition to the de-novo expression of IL-2R by activated peripheral blood T-cells, a released and fully soluble form of IL-2R (sIL-2R) has been detected. It was shown that sIL-2R is present in vivo, at low levels in the sera of healthy persons and at markedly elevated levels in various pathological conditions like neoplastic disorders. Soluble IL-2R levels are significantly elevated in the sera of patients with adult T-cell leukemia, hairy cell leukemia, lymphocytic leukemia, Hodgkin's and Non-Hodgkin's lymphoma, liver, breast and lung cancer. In patients with systemic lupus erythematosus, rheumatoid arthritis, progressive systemic sclerosis, polymyositis, Kawasaki disease, multiple sclerosis, and Diabetes type I plasma concentrations of sIL-2 receptor are significantly elevated as compared to controls.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CD25/IL-2R alpha ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.