Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82140

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

View all CD30/TNFRSF8 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CD30/TNFRSF8 in samples. No Significant cross-reactivity or interference between Rat CD30/TNFRSF8 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5%

Inter-Assay Precision (Precision between assays) CV% < 6%

Recovery for Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

Recovery

average = 91% for serum; average = 97% for EDTA plasma; average = 97% for cell culture media

Linearity

Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

Scientific Data Images for Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

ELISA: Rat CD30/TNFRSF8 ELISA Kit (Colorimetric) [NBP2-82140] -

ELISA: Rat CD30/TNFRSF8 ELISA Kit (Colorimetric) [NBP2-82140] - Standard Curve Reference

Kit Contents for Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD30/TNFRSF8

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Alternate Names

CD30, TNFRSF8

Gene Symbol

TNFRSF8

Additional CD30/TNFRSF8 Products

Product Documents for Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CD30/TNFRSF8 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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