CD31/PECAM-1 Antibody Pair [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79451
Key Product Details
Assay Type
Sandwich ELISA
Assay Range
18.75-12000 pg/ml (example only; lot dependent)
Sensitivity
18.75 pg/ml (example only; lot dependent)
Reactivity
Human
Product Specifications
Description
Solid Phase sandwich ELISA for the quantitative determination of Human CD31/PECAM-1.
Sample Volume Required
100 ul
Conjugate
HRP
Scientific Data Images for CD31/PECAM-1 Antibody Pair [HRP]
Sandwich ELISA: CD31/PECAM-1 Antibody Pair [HRP] [NBP2-79451] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Kit Contents for CD31/PECAM-1 Antibody Pair [HRP]
- Mouse Monoclonal Capture Antibody
- Mouse Monoclonal Detection Antibody (HRP-conjugated)
- Standard
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: CD31/PECAM-1
PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.
References
1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial
2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3
3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Alternate Names
CD31, EndoCAM, PECA1, PECAM-1, PECAM1
Gene Symbol
PECAM1
Additional CD31/PECAM-1 Products
Product Documents for CD31/PECAM-1 Antibody Pair [HRP]
Product Specific Notices for CD31/PECAM-1 Antibody Pair [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.
Loading...
Loading...
Loading...
Loading...