Human CD44 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86819

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine (20 uL)

Sensitivity

0.02 ng/mL

Assay Range

0.13 - 4.0 ng/mL

View all CD44 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay detects the standard form (CD44std). The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD44std positive serum. There was no crossreactivity detected, namely not with TNF alpha, TNF beta, TNF-R, IFN alpha 2c, IFN gamma, IL-8, Annexin, ELAM-1, L-selectin, ICAM-1 and HER-2.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.8%

Inter-Assay Precision (Precision between assays) CV% < 4.1%

Recovery for Human CD44 ELISA Kit (Colorimetric)

Recovery

The amount of endogenous human CD44std in unspiked serum was subtracted from the spike values. The recovery ranged from 76% to 101% with an overall mean recovery of 89%.

Scientific Data Images for Human CD44 ELISA Kit (Colorimetric)

ELISA: Human CD44 ELISA Kit (Colorimetric) [NBP1-86819]

ELISA: Human CD44 ELISA Kit (Colorimetric) [NBP1-86819]

ELISA: CD44 ELISA Kit [NBP1-86819] - Standard curve
ELISA: Human CD44 ELISA Kit (Colorimetric) [NBP1-86819]

ELISA: Human CD44 ELISA Kit (Colorimetric) [NBP1-86819]

ELISA: Human CD44 ELISA Kit (Colorimetric) [NBP1-86819] - The CD44std ELISA detects all circulating CD44 isoforms comprising the standard protein sequences. Standard protein sequences in black, variant exons, open boxes numbered v2 - v10.

Kit Contents for Human CD44 ELISA Kit (Colorimetric)

  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human C1q

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD44

CD44 (Pgp-1; Ly-24; ECMR III; F10-44-2; H-CAM; HUTCH-I; In(Lu)- related p80; Hermes antigen; hyaluronan receptor) is a polymorphic glycoprotein with apparent molecular weights ranging from 85 kDa to 250 kDa. This cell membrane associated molecule has a cytoplasmic tail (mediates the interaction with the cytoskeleton), a short hydrophobic transmembrane region and an NH2-terminal extracellular (binds to hyaluronate) domain. CD44 isoforms participate in a wide variety of cell-cell or cell-matrix interactions including lymphocyte homing, establishment of B and T cell immune responses, tumor metastases formation and inflammation. Three isoform categories of the CD44 molecule have been identified: - an 80-90 kDa isoform, the so-called standard form named CD44std, which is widely distributed on several hematopoietic and nonhemato- poietic cells including all subsets of leukocytes, monocytes, erythrocytes, many types of epithelium, mesenchymal elements like fibroblasts, smooth muscle cells and glial cells of the central nervous system, - a medium size category of 110-160 kDa, which is weakly expressed on epithelial cells and highly expressed in some carcinomas, and - a category that includes very large isoforms of 250 kDa covalently modified by the addition of chondroitin sulfate. These bigger isoforms of CD44 arise by alternative splicing of one or more "variant" exons (v2-v10) into the extracellular part of the 90kDa constant form molecule. Compared to the standard CD44, all larger isoforms are expressed in a much more restricted fashion, only in a few normal tissues or on the surface of certain tumor cells. Some splice variants of CD44 play important and distinct roles in tumor metastasis.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for Human CD44 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human CD44 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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