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Human CEBP Beta ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70025

Novus Biologicals, part of Bio-Techne
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NBP2-70025

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human C/EBP(beta) in samples. No significant cross-reactivity or interference between Human C/EBP(beta) and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.21%

Inter-Assay Precision (Precision between assays) CV% < 5.22%

Recovery for Human CEBP Beta ELISA Kit (Colorimetric)

Recovery

86-110%

Linearity

Human CEBP Beta ELISA Kit (Colorimetric)

Scientific Data Images for Human CEBP Beta ELISA Kit (Colorimetric)

Human CEBP Beta ELISA Kit (Colorimetric)

ELISA: Human CEBP Beta ELISA Kit (Colorimetric) [NBP2-70025] -

ELISA: Human CEBP Beta ELISA Kit (Colorimetric) [NBP2-70025] - Standard Curve Reference

Kit Contents for Human CEBP Beta ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CEBP Beta

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth and differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP beta occurs predominantly as a heterodimer (3). C/EBP beta gamma readily heterodimerize with each other as well as with C/EBP alpha (4). Additionally, 3 isoforms of C/EBP beta can be detected (LAP, LAP,LIP)

Alternate Names

C/EBP beta, C/EBP-beta, CCAAT/enhancer binding protein (C/EBP), beta, CCAAT/enhancer-binding protein beta, CRP2, IL6DBP, interleukin 6-dependent DNA-binding protein, LAPTCF-5, Liver activator protein, liver-enriched transcriptional activator protein, MGC32080, NF-IL6, Nuclear factor NF-IL6, nuclear factor of interleukin 6, TCF5NFIL6, Transcription factor 5

Gene Symbol

CEBPB

Additional CEBP Beta Products

Product Documents for Human CEBP Beta ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CEBP Beta ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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