Human CH25H - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31234

Novus Biologicals, part of Bio-Techne
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NBP3-31234
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.252 ng/mL (example only; lot dependent)

Assay Range

0.625 - 40 ng/mL (example only; lot dependent)

View all CH25H ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human CH25H - Ready-To-Use ELISA Kit (Colorimetric)

Human CH25H - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human CH25H  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31234] -

ELISA: Human CH25H - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31234] - Standard Curve Reference

Kit Contents for Human CH25H - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CH25H

CH25H that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq]

Alternate Names

C25H, cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, EC 1.14.99.38, h25OH

Gene Symbol

CH25H

Additional CH25H Products

Product Documents for Human CH25H - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CH25H - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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