Canine Clusterin/APOJ ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59978

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

4.963 ng/mL

Assay Range

7.813 ng/mL - 500 ng/mL

View all Clusterin ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Canine

Specificity

This assay has high sensitivity and excellent specificity for detection of dog clusterin. No significant cross-reactivity or interference between dog clusterin and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Canine Clusterin/APOJ ELISA Kit (Colorimetric)

ELISA: Canine Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-59978]

ELISA: Canine Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-59978]

ELISA: Canine Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-59978] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Canine Clusterin/APOJ ELISA Kit (Colorimetric)

Canine Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-59978] -

Canine Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-59978] - Standard Curve Reference

Kit Contents for Canine Clusterin/APOJ ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Clusterin

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with clusterin. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for clusterin. The competitive inhibition reaction is launched between with pre-coated clusterin and clusterin in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of clusterin in the samples. The color development is stopped and the intensity of the color is measured.

Alternate Names

APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2

Gene Symbol

CLU

Additional Clusterin Products

Product Documents for Canine Clusterin/APOJ ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Canine Clusterin/APOJ ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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