Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82491

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Coagulation Factor II/Thrombin ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Coagulation Factor II/Thrombin in samples. No Significant cross-reactivity or interference between Rat Coagulation Factor II/Thrombin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.15

Inter-Assay Precision (Precision between assays) CV% < 4.46

Recovery for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Recovery

85-106%

Linearity

Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Scientific Data Images for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

ELISA: Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) [NBP2-82491] -

ELISA: Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) [NBP2-82491] - Standard Curve Reference

Kit Contents for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor II/Thrombin

Proteinase-activated receptor 3 (PAR3) is a member of the Proteinase-Activated Receptor subfamily. The enzyme thrombin is involved in the activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of PAR3 and other receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR3 expression has been documented in blood, bone marrow, lung, spleen, and vessel. ESTs have been isolated from head/neck, skeletal muscle, and skin libraries.

Alternate Names

F2, Thrombin

Gene Symbol

F2

Additional Coagulation Factor II/Thrombin Products

Product Documents for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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