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Rat Coagulation Factor VII ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82480

Novus Biologicals, part of Bio-Techne
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NBP2-82480

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

37.5 pg/mL

Assay Range

62.5 - 4000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Coagulation Factor VII in samples. No Significant cross-reactivity or interference between Rat Coagulation Factor VII and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.6

Inter-Assay Precision (Precision between assays) CV% < 5.29

Recovery for Rat Coagulation Factor VII ELISA Kit (Colorimetric)

Recovery

84-108%

Linearity

Rat Coagulation Factor VII ELISA Kit (Colorimetric)

Scientific Data Images for Rat Coagulation Factor VII ELISA Kit (Colorimetric)

Rat Coagulation Factor VII ELISA Kit (Colorimetric)

ELISA: Rat Coagulation Factor VII ELISA Kit (Colorimetric) [NBP2-82480] -

ELISA: Rat Coagulation Factor VII ELISA Kit (Colorimetric) [NBP2-82480] - Standard Curve Reference

Kit Contents for Rat Coagulation Factor VII ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor VII

Factor VII is a 50kDa multi-domain, single chain plasma glycoprotein synthesized in the liver and having abnormal circulating concentration of between 400 and 600 ug/L. It is essential for blood coagulation. Factor VII is converted to the active, two chain, serine protease Factor VIIa through cleavage by thrombin, factor Xa, factor IXa or factor XIIa. Factor VIIa converts factor X to factor Xa, which in turn converts prothrombin to thrombin.

Long Name

Coagulation Factor VII (Serum Prothrombin Conversion Accelerator)

Alternate Names

F7

Gene Symbol

F7

Additional Coagulation Factor VII Products

Product Documents for Rat Coagulation Factor VII ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Coagulation Factor VII ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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