Human Collagen II ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75853

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63 - 40 ng/mL

View all Collagen II ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human COL2A1 in samples. No significant cross-reactivity or interference between Human COL2A1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.04

Inter-Assay Precision (Precision between assays) %CV < 4.76

Recovery for Human Collagen II ELISA Kit (Colorimetric)

Recovery

85 - 101%

Linearity

Human Collagen II ELISA Kit (Colorimetric)

Scientific Data Images for Human Collagen II ELISA Kit (Colorimetric)

Human Collagen II ELISA Kit (Colorimetric)

ELISA: Human Collagen II ELISA Kit (Colorimetric) [NBP2-75853] -

ELISA: Human Collagen II ELISA Kit (Colorimetric) [NBP2-75853] - Standard Curve Reference

Kit Contents for Human Collagen II ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Collagen II

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Gene Symbol

COL2A1

Additional Collagen II Products

Product Documents for Human Collagen II ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Collagen II ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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