Human Collagen IV alpha 1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75870

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Collagen IV alpha 1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human COL4a1 in samples. No significant cross-reactivity or interference between Human COL4a1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.92%

Inter-Assay Precision (Precision between assays) CV% < 5.49%

Recovery for Human Collagen IV alpha 1 ELISA Kit (Colorimetric)

Recovery

87-109%

Linearity

Human Collagen IV alpha 1 ELISA Kit (Colorimetric)

Scientific Data Images for Human Collagen IV alpha 1 ELISA Kit (Colorimetric)

Human Collagen IV alpha 1 ELISA Kit (Colorimetric)

ELISA: Human Collagen IV alpha 1 ELISA Kit (Colorimetric) [NBP2-75870] -

ELISA: Human Collagen IV alpha 1 ELISA Kit (Colorimetric) [NBP2-75870] - Standard Curve Reference

Kit Contents for Human Collagen IV alpha 1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Collagen IV alpha 1

Collagen IV is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Alternate Names

Arresten, COL4A1

Gene Symbol

COL4A1

Additional Collagen IV alpha 1 Products

Product Documents for Human Collagen IV alpha 1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Collagen IV alpha 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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