Rat Collagen IV ELISA Kit (Chemiluminescence)

Name: Rat Collagen IV ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP2-75863

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75863

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

56.25 pg/mL

Assay Range

93.75-6000 pg/mL

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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Rat

Specificity

This kit recognizes Rat COL4 in samples. No significant cross-reactivity or interference between Rat COL4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.1%

Inter-Assay Precision (Precision between assays) CV% < 8.69%

Recovery for Rat Collagen IV ELISA Kit (Chemiluminescence)

Recovery

88-110%

Linearity

Scientific Data Images for Rat Collagen IV ELISA Kit (Chemiluminescence)

ELISA: Rat Collagen IV ELISA Kit (Chemiluminescence) [NBP2-75863] -

ELISA: Rat Collagen IV ELISA Kit (Chemiluminescence) [NBP2-75863] - Standard Curve Reference

Kit Contents for Rat Collagen IV ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Collagen IV

Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

Additional Collagen IV Products

Product Documents for Rat Collagen IV ELISA Kit (Chemiluminescence)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Rat Collagen IV ELISA Kit (Chemiluminescence) Manual

COA

Product Specific Notices for Rat Collagen IV ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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