Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75877

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL

View all Collagen VI alpha 1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human COL6a1 in samples. No significant cross-reactivity or interference between Human COL6a1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.78%

Inter-Assay Precision (Precision between assays) CV% < 8.27%

Recovery for Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)

Recovery

86-113%

Linearity

Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)

Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)

ELISA: Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence) [NBP2-75877] -

ELISA: Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence) [NBP2-75877] - Standard Curve Reference

Kit Contents for Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Collagen VI alpha 1

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

Alternate Names

Collagen 6, collagen alpha-1(VI) chain, collagen VI, alpha-1 polypeptide, collagen, type VI, alpha 1, Collagen-6, human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA), OPLL

Gene Symbol

COL6A1

Additional Collagen VI alpha 1 Products

Product Documents for Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Collagen VI alpha 1 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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