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Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82142

Novus Biologicals, part of Bio-Techne
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NBP2-82142

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat COMP/Thrombospondin-5 in samples. No significant cross-reactivity or interference between Rat COMP/Thrombospondin-5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Recovery

average = 101% for serum; average = 99% for EDTA plasma; average = 93% for cell culture media

Linearity

Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Scientific Data Images for Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

ELISA: Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82142] -

ELISA: Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric) [NBP2-82142] - Standard Curve Reference

Kit Contents for Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: COMP/Thrombospondin-5

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Gene Symbol

COMP

Additional COMP/Thrombospondin-5 Products

Product Documents for Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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