Human Cortisol ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18003

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 uL)

Sensitivity

2.92 ng/mL

Assay Range

6.25-400 ng/mL

View all Cortisol ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8.78

Inter-Assay Precision (Precision between assays) %CV < 8.96

Recovery for Human Cortisol ELISA Kit (Colorimetric)

Recovery

81-100%

Linearity

Human Cortisol ELISA Kit (Colorimetric)

Scientific Data Images for Human Cortisol ELISA Kit (Colorimetric)

Human Cortisol ELISA Kit (Colorimetric)

ELISA: Human Cortisol ELISA Kit (Colorimetric) [NBP3-18003] -

ELISA: Human Cortisol ELISA Kit (Colorimetric) [NBP3-18003] - Standard Curve Reference

Kit Contents for Human Cortisol ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cortisol

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Alternate Names

Compound F, Hydrocortisone

Additional Cortisol Products

Product Documents for Human Cortisol ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Cortisol ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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