Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0554

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Plasma (EDTA), Serum (100 uL)

Sensitivity

<1 pg/mL

Assay Range

15.6 to 1000 pg/mL

View all CXCL1/GRO alpha/KC/CINC-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Description

Cxcl1 (Rat) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of rat Cxcl1.

Kit Contents for Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Kit (Colorimetric)

  • ABC diluent buffer
  • Antibody diluent buffer
  • Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  • Biotinylated anti- rat CXCL1 antibody, dilution 1:100
  • Lyophilized recombinant rat CXCL1 standard
  • One 96-well plate precoated with anti- rat CXCL1 antibody
  • Sample diluent buffer
  • TMB color developing agent
  • TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Gene Symbol

CXCL1

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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