Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11823

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all CXCL12/SDF-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Rat SDF-1/CXCL12 and analogues was observed.

Description

This ELISA kit applies to the in vitro quantitative determination of Rat SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.62

Inter-Assay Precision (Precision between assays) %CV < 5.38

Recovery for Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

Recovery

83 - 106%

Linearity

Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

ELISA: Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-11823] -

ELISA: Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-11823] - Standard Curve Reference

Kit Contents for Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Antibody Diluent
  • Concentrated Biotinylated Detection Antibody
  • Concentrated HRP Conjugate
  • Concentrated Wash Buffer
  • HRP Conjugate Diluent
  • Micro ELISA Plate
  • Plate Sealer
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CXCL12/SDF-1

The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1. Three isoforms are known for mouse and rat.

Alternate Names

PBSF, SDF-1, SDF1

Gene Symbol

CXCL12

Additional CXCL12/SDF-1 Products

Product Documents for Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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