Rat CXCL5/ENA-78 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78751

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

View all CXCL5/ENA-78 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.10%

Inter-Assay Precision (Precision between assays) CV% < 4.95%

Recovery for Rat CXCL5/ENA-78 ELISA Kit (Colorimetric)

Recovery

88-106%

Linearity

Rat CXCL5/ENA-78 ELISA Kit (Colorimetric)

Scientific Data Images for Rat CXCL5/ENA-78 ELISA Kit (Colorimetric)

Rat CXCL5/ENA-78 ELISA Kit (Colorimetric)

ELISA: Rat CXCL5/ENA-78 ELISA Kit (Colorimetric) [NBP2-78751] -

ELISA: Rat CXCL5/ENA-78 ELISA Kit (Colorimetric) [NBP2-78751] - Standard Curve Reference

Kit Contents for Rat CXCL5/ENA-78 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CXCL5/ENA-78

CXCL5/Epithelial Cell-derived Neutrophil-activating Peptide (ENA-78), is a member of the CXC subfamily of chemokines. Full-length CXCL5/ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. Following the removal of the signal peptide, bioactive CXCL5/ENA-78 is 78 aa in length. CXCL5/ENA-78 can be N-terminally cleaved by Cathepsin G and Chymotrypsin to CXCL5/ENA-74 (74 aa) and CXCL5/ENA-70 (70 aa), which show increased potency relative to CXCL5/ENA-78. While murine LIX was thought to be an ortholog to human CXCL5/ENA-78, genome-wide analysis and a consensus in the field suggests that human CXCL5/ENA-78 does not have a true murine ortholog. CXCL5/ENA-78 is upregulated at sites of inflammation and is expressed by multiple hematopoietic cell types, fibroblasts, endothelial cells, vascular smooth muscle cells, and adipocytes. It acts as a chemoattractant for neutrophils, has angiogenic properties, and contributes to cancer progression.

Alternate Names

CXCL5, ENA-78, ENA78, ENA-78/CXCL5, LIX/CXCL5

Gene Symbol

CXCL5

UniProt

P97885

Additional CXCL5/ENA-78 Products

Product Documents for Rat CXCL5/ENA-78 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CXCL5/ENA-78 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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