I want to carry out a research using cystatin c to determine GFR in sickle cell anemia patients. Can pin prick sample be used? What is the procedure to carry out the test?

Name: Human, Monkey Cystatin C ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA0022

The cystatin C ELISA kits we sell are manufactured by two companies, Abnova and Biosensis, and distributed by us here at Novus Biologicals. I contacted both companies to ask them your question. I have now heard back from Biosensis, who produce the product catalog # BEK-2145-2P. Their answer was as follows: We haven't used our kit with such samples; however, a recent paper suggests that it could be possible. The paper used a different method than traditional ELISA, but if the samples were allowed to clot or collected in citrated tubes it certainly seems possible to use such samples, but the customer will have to investigate on their own the true effectiveness compared to traditional blood sample collection, for which the kit is tested. Here is a link to the paper examining capillary tube collection (https://academic.oup.com/clinchem/article/51/5/903/5629843) These people even have a patent on using the technology for the measure of cystatin C for human samples (http://helierscientific.co.uk/renalfunction.html) so that too might be an issue for your customer. Finally, this paper too probably should be reviewed by your customer; it is more modern and discusses a number of approaches and issues (https://journals.lww.com/cjasn/pages/articleviewer.aspx?year=2009&issue=11000&article=00029&type=Fulltext) Just to let you know that I have now heard from Abnova, the manufacturer of our cystatin C ELISA kits product catalog # KA0022 (protocol: http://www.abnova.com/protocol_pdf/KA0022.pdf) and catalog # KA2107 (protocol: http://www.abnova.com/protocol_pdf/KA2107.pdf). Their response was simply that neither of the kits have been tested on pin-prick blood samples, and so they are not sure whether these kits are suitable or not.

Human, Monkey Cystatin C ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0022

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cerebrospinal Fluid, Plasma (Citrate, EDTA, Heparin), Serum, Urine (100 uL (diluted))

Sensitivity

0.25 ng/mL

Assay Range

200 to 10000 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Monkey

Description

Quality control test: Standard curve. CST3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human cystatin C.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.30%

Inter-Assay Precision (Precision between assays) CV% < 5.50%

Recovery for Human, Monkey Cystatin C ELISA Kit (Colorimetric)

Recovery

90.80%

Scientific Data Images for Human, Monkey Cystatin C ELISA Kit (Colorimetric)

ELISA: Human, Monkey Cystatin C ELISA Kit (Colorimetric) [KA0022]

ELISA: Cystatin C ELISA Kit [HRP] [KA0022] - Typical Standard Curve for CST3 (Human) ELISA Kit.

Kit Contents for Human, Monkey Cystatin C ELISA Kit (Colorimetric)

Antibody Coated Microtiter Strips
Conjugate Diluent (ready to use)
Conjugate Solution Conc. (50x)
Dilution Buffer Conc. (10 x)
Quality Control HIGH (concentrated)
Quality Control LOW (concentrated)
Set of Standards (concentrated)
Stop Solution (ready to use)
Substrate Solution (ready to use)
Wash Solution Conc. (10 x)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cystatin C

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.

Alternate Names

ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin

Gene Symbol

CST3

Additional Cystatin C Products

Product Documents for Human, Monkey Cystatin C ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download CST3 (Human) ELISA Kit

COA

SDS

Download SDS

Product Specific Notices for Human, Monkey Cystatin C ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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