Human Cytochrome c ELISA Kit (Colorimetric)

Name: Human Cytochrome c ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-91263

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91263

Submit a review

Product Datasheet / COA / SDS / Protocols & Manuals

100% Guarantee

Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture lysates (100 uL (prediluted))

Sensitivity

0.05 ng/mL

Assay Range

0.08 - 5.0 ng/mL

View all Cytochrome c ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Cytochrome c positive sample. There was no cross reactivity detected.

Description

: The human Cytochrome c ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Cytochrome c.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.00%

Inter-Assay Precision (Precision between assays) CV% < 4.00%

Recovery for Human Cytochrome c ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 78% to 88% with an overall mean recovery of 82%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human Cytochrome c ELISA Kit (Colorimetric)

Human Cytochrome c ELISA Kit [NBP1-91263] - Data for Human Cytochrome c Platinum ELISA

Kit Contents for Human Cytochrome c ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch with a Microwell Plate coated with monoclonal antibody to human Cytochrome c
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Biotin-Conjugate anti-human Cytochrome c monoclonal antibody
Human Cytochrome c Standard lyophilized, 10 ng/ml upon reconstitution
Lysis Buffer 10x
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cytochrome c

Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells. Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria. Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Moreover, elevated cytochrome c levels were observed in serum from patients with hematological malignancies. In the course of cancer chemotherapy, the serum-cytochrome c level grew rapidly and it decreased gradually as the patient was cleared from malignant cells. Thus, serum-cytochrome c monitoring might serve as a clinical marker indicating the onset of apoptosis and cell turn-over in vivo.

Alternate Names

CYCS

Gene Symbol