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Human Desert Hedgehog/Dhh ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31885

Novus Biologicals, part of Bio-Techne
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NBP3-31885

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.052 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Desert Hedgehog/Dhh ELISA Kit (Colorimetric)

Human Desert Hedgehog/Dhh ELISA Kit (Colorimetric)

ELISA: Human Desert Hedgehog/Dhh ELISA Kit (Colorimetric) [NBP3-31885] -

ELISA: Human Desert Hedgehog/Dhh ELISA Kit (Colorimetric) [NBP3-31885] - Standard Curve Reference

Kit Contents for Human Desert Hedgehog/Dhh ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Desert Hedgehog/Dhh

Dhh encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq]

Alternate Names

DHH

Gene Symbol

DHH

Additional Desert Hedgehog/Dhh Products

Product Documents for Human Desert Hedgehog/Dhh ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Desert Hedgehog/Dhh ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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