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Rat Dkk-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82461

Novus Biologicals, part of Bio-Techne
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NBP2-82461

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Dkk-1 in samples. No Significant cross-reactivity or interference between Rat Dkk-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.7

Inter-Assay Precision (Precision between assays) CV% < 4.63

Recovery for Rat Dkk-1 ELISA Kit (Colorimetric)

Recovery

86-109%

Linearity

Rat Dkk-1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Dkk-1 ELISA Kit (Colorimetric)

Rat Dkk-1 ELISA Kit (Colorimetric)

ELISA: Rat Dkk-1 ELISA Kit (Colorimetric) [NBP2-82461] -

ELISA: Rat Dkk-1 ELISA Kit (Colorimetric) [NBP2-82461] - Standard Curve Reference

Kit Contents for Rat Dkk-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Dkk-1

DKK1 is a secreted factor that has been linked to the function of osteoblasts. Elevated DKK1 levels in bone marrow plasma and peripheral blood from patients with multiple myeloma correlated with the gene-expression patterns of DKK1 and were associated with the presence of focal bone lesions. The production of DKK1, an inhibitor of osteoblast differentiation, by myeloma cells is associated with the presence of lytic bone lesions in patients with multiple myeloma.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Gene Symbol

DKK1

Additional Dkk-1 Products

Product Documents for Rat Dkk-1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Dkk-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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