Human DNASE1 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18213
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and cell culture supernates (100 ul)
Sensitivity
0.03 ng/ml
Assay Range
1.56-100 ng/ml
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human DNASE1 in the sample, this Human DNASE1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human DNASE1 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10
Inter-Assay Precision (Precision between assays) %CV < 15
Recovery for Human DNASE1 ELISA Kit (Colorimetric)
Recovery
80-118%
Scientific Data Images for Human DNASE1 ELISA Kit (Colorimetric)
![ELISA: Human DNASE1 ELISA Kit (Colorimetric) [NBP3-18213]](https://resources.bio-techne.com/images/products/Human-DNASE1-ELISA-Kit-Colorimetric-ELISA-NBP3-18213-img0001.jpg "ELISA: Human DNASE1 ELISA Kit (Colorimetric) [NBP3-18213]")
ELISA: Human DNASE1 ELISA Kit (Colorimetric) [NBP3-18213]
ELISA: Human DNASE1 ELISA Kit (Colorimetric) [NBP3-18213] - Standard Curve ReferenceKit Contents for Human DNASE1 ELISA Kit (Colorimetric)
- Balance Solution
- Enzyme Conjugate
- Microtiter Plate
- Standards
- Stop Solution
- Substrate A
- Substrate B
- Wash Solution
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: DNASE1
Alternate Names
deoxyribonuclease IFLJ38093, deoxyribonuclease-1, DKFZp686H0155, DNase I, DNase I, lysosomal, DNL1FLJ44902, Dornase alfa, DRNI, EC 3.1.21.1, human urine deoxyribonuclease I
Gene Symbol
DNASE1
Additional DNASE1 Products
Product Documents for Human DNASE1 ELISA Kit (Colorimetric)
Product Specific Notices for Human DNASE1 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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