Human Dynamin ELISA Kit (Colorimetric)

Name: Human Dynamin ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-31466

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31466

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.112 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Dynamin ELISA Kit (Colorimetric)

ELISA: Human Dynamin ELISA Kit (Colorimetric) [NBP3-31466] -

ELISA: Human Dynamin ELISA Kit (Colorimetric) [NBP3-31466] - Standard Curve Reference

Kit Contents for Human Dynamin ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Dynamin

The dynamin family of GTPases is essential for receptor-mediated endocytosis and synaptic vesicle recycling, and it has recently been shown to play a role in vesicle formation from the trans-Golgi network (1). Dynamin-1 is a GTP-, microtubule-, and phospholipid-binding protein that is expressed primarily in brain and almost exclusively expressed in the central nervous system (2). Dynamin-1 is believed to assemble around the necks of clathrin-coated pits and assist in pinching vesicles from the plasma membrane. This role would make dynamin unique among GTPases in its ability to act as a mechanochemical enzyme (1). Dynamin-1 function is apparently essential for scission of newly formed vesicles from the plasma membrane; it has been proposed that dynamin is a regulator of downstream effectors of scission, rather than being directly responsible for it (3).

Alternate Names

DNM1

Gene Symbol